2014年1月6日星期一

Histologic Evaluation of Laser Lipolysis Pulsed 1064-nmNd YAG Laser vs CW980nm Diode Laser

Keywords:Laser Lipolysis Pulsed 1064-nmNd YAG Laser,CW980nm Diode Laser

Background: The use of the laser as an auxiliary tool has refined the. traditional technique for lipoplasty. During laser lipolysis, the interaction between the lasers and the fat produced direct cellular destruction before the suction, reduced bleeding, and promoted skin tightening

Objective: This study sought to perform  a comparative bistologic evaluation of laser lipolysis with the pulsed 1064-nm NA:YAC laser versus a continuour 980-nm diode laser.

Methods: A pulsed 1064-nm Nd:YAG and a CW 980-nm diode laser were evaluated at different energy settings for lipolysis on the thights of a fresh acdaver.The laser were coupled to 600-um optical fiber inserted in‘1-mm diameter cnnula. Biopsy specimens were taken on irradiated and non-irradiated areas.Hematoxylin-erythrosin-safran staining and immunostaining (anti-PS100 polyclonal antibody) were performed to identify fat tissue damage.

Results: In the absence of laser exposures (control specimens), cavitirs created by cannulation were seen; adipocytes were round in appearance and not deflated.At low energy settings, tumescent adipocytes were observed. At higher energy settings,cytoplasmic retraction,disruption of mermbranes, and heat-coagulated collagen fibers were noted; coagulated blood cells were also present. For the highest energy settings, carbonization of fat tissue involving fibers and membranes was clearly seen. for equivalent energy settings,  1064-nm and 980-nm  wavelengths gave smiler histologic  results.


Condusiora: Laser lipolysis is relatively new technique that is still under development. Our histologic findings suggest several positive benefits of the Iaser, including skin retraction and n reduction in intraoperative bleeding. The interaction of the laser with the tissue is similar at 980 nm and 1064 nm with the some energy settings. Because higher volumes of fat are removed with higher total energy,a high-power 980-nm diode laser could offer an interesting alternative to the 1064-nm Nd:YAG laser. 

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